Toluidine blue stain

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Toluidine blue is a basic thiazine metachromatic dye with high affinity for acidic tissue components.[1]

It stains nucleic acids blue and polysaccharides purple and also increases the sharpness of histology slide images. It is especially useful today for staining chromosomes in plant or animal tissues, as a replacement for Aceto-orcein stain.

Results

The results depend on the studied organs:[2]

- Mastocytes in purple

- Cartilage in purple

- Mucins in purple/red

- Nuclei in blue

It is used in forensic examination,[3] renal pathology[4] and neuropathology.

See also

References

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External links

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